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Establishment of the GSC–neuron coculture. ( a ) Neuron differentiation: iPSC-derived <t>NPCs</t> were induced to differentiate into neurons for three weeks and were characterized for the neuronal markers Tuj1 (green) and NeuN (red). Cell nuclei were stained with DAPI (blue). ( b ) Establishment of the GSC–neuron coculture: NeuN + neurons were used for the cocultures. GFP + GSCs (NSC11 and NSC20) were added directly to the neurons in the GSC media for 48 h to establish the coculture. The figure shows representative images of the GSCs (GFP: green)–neurons (NeuN: red) in the direct coculture. The images were captured with a confocal microscope using a 40× lens, and images of the neurons are presented as orthogonal projections.
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Establishment of the GSC–neuron coculture. ( a ) Neuron differentiation: iPSC-derived <t>NPCs</t> were induced to differentiate into neurons for three weeks and were characterized for the neuronal markers Tuj1 (green) and NeuN (red). Cell nuclei were stained with DAPI (blue). ( b ) Establishment of the GSC–neuron coculture: NeuN + neurons were used for the cocultures. GFP + GSCs (NSC11 and NSC20) were added directly to the neurons in the GSC media for 48 h to establish the coculture. The figure shows representative images of the GSCs (GFP: green)–neurons (NeuN: red) in the direct coculture. The images were captured with a confocal microscope using a 40× lens, and images of the neurons are presented as orthogonal projections.
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Establishment of the GSC–neuron coculture. ( a ) Neuron differentiation: iPSC-derived <t>NPCs</t> were induced to differentiate into neurons for three weeks and were characterized for the neuronal markers Tuj1 (green) and NeuN (red). Cell nuclei were stained with DAPI (blue). ( b ) Establishment of the GSC–neuron coculture: NeuN + neurons were used for the cocultures. GFP + GSCs (NSC11 and NSC20) were added directly to the neurons in the GSC media for 48 h to establish the coculture. The figure shows representative images of the GSCs (GFP: green)–neurons (NeuN: red) in the direct coculture. The images were captured with a confocal microscope using a 40× lens, and images of the neurons are presented as orthogonal projections.
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Establishment of the GSC–neuron coculture. ( a ) Neuron differentiation: iPSC-derived <t>NPCs</t> were induced to differentiate into neurons for three weeks and were characterized for the neuronal markers Tuj1 (green) and NeuN (red). Cell nuclei were stained with DAPI (blue). ( b ) Establishment of the GSC–neuron coculture: NeuN + neurons were used for the cocultures. GFP + GSCs (NSC11 and NSC20) were added directly to the neurons in the GSC media for 48 h to establish the coculture. The figure shows representative images of the GSCs (GFP: green)–neurons (NeuN: red) in the direct coculture. The images were captured with a confocal microscope using a 40× lens, and images of the neurons are presented as orthogonal projections.
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Establishment of the GSC–neuron coculture. ( a ) Neuron differentiation: iPSC-derived <t>NPCs</t> were induced to differentiate into neurons for three weeks and were characterized for the neuronal markers Tuj1 (green) and NeuN (red). Cell nuclei were stained with DAPI (blue). ( b ) Establishment of the GSC–neuron coculture: NeuN + neurons were used for the cocultures. GFP + GSCs (NSC11 and NSC20) were added directly to the neurons in the GSC media for 48 h to establish the coculture. The figure shows representative images of the GSCs (GFP: green)–neurons (NeuN: red) in the direct coculture. The images were captured with a confocal microscope using a 40× lens, and images of the neurons are presented as orthogonal projections.
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Establishment of the GSC–neuron coculture. ( a ) Neuron differentiation: iPSC-derived <t>NPCs</t> were induced to differentiate into neurons for three weeks and were characterized for the neuronal markers Tuj1 (green) and NeuN (red). Cell nuclei were stained with DAPI (blue). ( b ) Establishment of the GSC–neuron coculture: NeuN + neurons were used for the cocultures. GFP + GSCs (NSC11 and NSC20) were added directly to the neurons in the GSC media for 48 h to establish the coculture. The figure shows representative images of the GSCs (GFP: green)–neurons (NeuN: red) in the direct coculture. The images were captured with a confocal microscope using a 40× lens, and images of the neurons are presented as orthogonal projections.
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Establishment of the GSC–neuron coculture. ( a ) Neuron differentiation: iPSC-derived <t>NPCs</t> were induced to differentiate into neurons for three weeks and were characterized for the neuronal markers Tuj1 (green) and NeuN (red). Cell nuclei were stained with DAPI (blue). ( b ) Establishment of the GSC–neuron coculture: NeuN + neurons were used for the cocultures. GFP + GSCs (NSC11 and NSC20) were added directly to the neurons in the GSC media for 48 h to establish the coculture. The figure shows representative images of the GSCs (GFP: green)–neurons (NeuN: red) in the direct coculture. The images were captured with a confocal microscope using a 40× lens, and images of the neurons are presented as orthogonal projections.
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Establishment of the GSC–neuron coculture. ( a ) Neuron differentiation: iPSC-derived <t>NPCs</t> were induced to differentiate into neurons for three weeks and were characterized for the neuronal markers Tuj1 (green) and NeuN (red). Cell nuclei were stained with DAPI (blue). ( b ) Establishment of the GSC–neuron coculture: NeuN + neurons were used for the cocultures. GFP + GSCs (NSC11 and NSC20) were added directly to the neurons in the GSC media for 48 h to establish the coculture. The figure shows representative images of the GSCs (GFP: green)–neurons (NeuN: red) in the direct coculture. The images were captured with a confocal microscope using a 40× lens, and images of the neurons are presented as orthogonal projections.
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Establishment of the GSC–neuron coculture. ( a ) Neuron differentiation: iPSC-derived NPCs were induced to differentiate into neurons for three weeks and were characterized for the neuronal markers Tuj1 (green) and NeuN (red). Cell nuclei were stained with DAPI (blue). ( b ) Establishment of the GSC–neuron coculture: NeuN + neurons were used for the cocultures. GFP + GSCs (NSC11 and NSC20) were added directly to the neurons in the GSC media for 48 h to establish the coculture. The figure shows representative images of the GSCs (GFP: green)–neurons (NeuN: red) in the direct coculture. The images were captured with a confocal microscope using a 40× lens, and images of the neurons are presented as orthogonal projections.

Journal: Cancers

Article Title: Glioblastoma Cells Induce Neuron Loss In Vivo and In Vitro

doi: 10.3390/cancers17172817

Figure Lengend Snippet: Establishment of the GSC–neuron coculture. ( a ) Neuron differentiation: iPSC-derived NPCs were induced to differentiate into neurons for three weeks and were characterized for the neuronal markers Tuj1 (green) and NeuN (red). Cell nuclei were stained with DAPI (blue). ( b ) Establishment of the GSC–neuron coculture: NeuN + neurons were used for the cocultures. GFP + GSCs (NSC11 and NSC20) were added directly to the neurons in the GSC media for 48 h to establish the coculture. The figure shows representative images of the GSCs (GFP: green)–neurons (NeuN: red) in the direct coculture. The images were captured with a confocal microscope using a 40× lens, and images of the neurons are presented as orthogonal projections.

Article Snippet: iPSC-derived NPCs (Catalog #ACS5004) were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Derivative Assay, Staining, Microscopy